Quantification of molecular interactions by KinExA technology

Biological processes include sequences of interactions between biomolecular species, which occur in all living systems as essential components of the cellular machinery, and quantitative approaches are widely recognized as pivotal for gaining a deeper understanding of the relationships between complex interactions in biological systems and the emerging functional properties. The KinExA (Kinetics Exclusion Assay) technology is increasingly recognized as a leader for providing ultra-accurate analyses of molecular interactions in true solution phase systems. In this respect, this presentation will begin with a short description of the basic principles of the KinExA technology for quantifying bi-molecular interactions through specifically assessing only the non-occupied binding sites in an equilibrated mixture containing both occupied and non-occupied sites. A brief description of work published elsewhere on applications of the technology for equilibrium, concentration, and kinetics measurements will be provided. Next, the presentation will emphasize current advancements of the technology for quantification of membrane-ligand interactions in membrane systems reconstituted on a solid, spherical support. The conclusive remarks will include both advantages and limitations of KinExA over other current technologies and instruments.